Hoefer SP-2001 Vision Life Spectrophotometer Manuel d'utilisateur

Vision Spectrophotometer USER MANUAL

Version 1.0 Page 10 1: Single Wavelength – Abs and %T This makes simple Absorbance (A) and % Transmission (%T) measurements on samples, measu

Version 1.0 Page 11 Options (select using key pad numbers) 1. Return to parameters screen (step 1 above).

Version 1.0 Page 12 2: Concentration This makes simple concentration measurements on samples, by measuring the amount of light that has passed

Version 1.0 Page 13 Step 6 (if using standard mode) Insert the reference. Press 0A/100% key. This will be used for all subsequent

Version 1.0 Page 14 3: Wavescan An absorption spectrum can be obtained from your instrument, enabling simple identification of peak height and

Version 1.0 Page 15 Options (select using key pad numbers) 1. Return to parameters screen (ste

Version 1.0 Page 16 Add Peak... (Shortcut button 5) Adds a used defined peak at the current cursor position.

Version 1.0 Page 17 4: Simple Kinetics Kinetics studies, where the change in absorbance needs to be followed as a function of time at a fixed

Version 1.0 Page 18 ResultsInsert the reference and press the 0A/100%T key. Insert the sample and press to s

Version 1.0 Page 19 Options (select using key pad numbers) 1. Return to parameter 1 screen (step 1 above). 2. Print data on the res

Version 1.0 Page 2 Declaration of Conformity This is to certify that the Vision UV/Visible Spectrophotometers part numbers: SP-2001,

Version 1.0 Page 20 5: Standard Curve The construction of a multi-point calibration curve from standards of known concentration to quantify un

Version 1.0 Page 21 Standards screen Step 8 Enter the concentration values by using the keypad numbers and the up and down arrow

Version 1.0 Page 22 Calibration Screen (replicates on) This shows the calibration values and allows standards to be measured. Step 10 Inse

Version 1.0 Page 23 Options (select using key pad numbers) 1. Return to parameters screen (step 1 above). 2. Print result

Version 1.0 Page 24 6: Multiple Wavelength This makes up to 5 absorbance measurements on the same sample. The procedure is as follows: S

Version 1.0 Page 25 7: Absorbance Ratio This makes simple Absorbance ratio measurements on samples, measuring the amount of light that has pas

Version 1.0 Page 26 Results ScreenStep 10 Insert the reference. Press 0A/100% key. This will be used for all subsequent samples until chan

Version 1.0 Page 27 THE LIFE SCIENCE FOLDER Life Science Folder This contains three sub folders; Nucleic Acids, Protein and Cell Count. Cont

Version 1.0 Page 28 Use of Background Correction • Background correction at a wavelength totally separate from the nucleic acid and protein

Version 1.0 Page 29 1: DNA The procedure is as follows: Step 1Press 1 to select DNA mode. Step 2 Select path length using the left and right a

Version 1.0 Page 3 TABLE OF CONTENTS ESSENTIAL SAFETY NOTES ...

Version 1.0 Page 30 Options (select using key pad numbers) 1. Return to parameters screen (step 1 above). 2. Print result via selected

Version 1.0 Page 31 2: RNA The procedure is as follows: Step 1Press 2 to select RNA mode. Step 2 Select path length using the left and right

Version 1.0 Page 32 Options (select using key pad numbers) 1. Return to parameters screen (step 1 above). 2. Print result via se

Version 1.0 Page 33 3: Oligo The procedure is as follows: Step 1Press 3 to select Oligo mode. Step 2 Select path length using the left and

Version 1.0 Page 34 Results ScreenStep 8 Insert the reference. Press 0A/100%T Key. This will be used for all subsequent

Version 1.0 Page 35 Protein Determination Protein Determination at 280 nm • Protein can be determined at 280 nm due to absorption by tyrosine

Version 1.0 Page 36 1: Protein UV This is the Christian and Warburg assay discussed previously. The procedure is as follows: Step 1Press 1 to

Version 1.0 Page 37 Results ScreenStep 9 Insert the reference. Press 0A/100%T Key. This will be used for all subsequent samples

Version 1.0 Page 38 2: BCA The procedure is as follows: Step 1Press 2 to select BCA mode. S

Version 1.0 Page 39 Calibration Screen (replicates off) This shows the calibration values and allows standards to be measured. Step 11 Insert

Version 1.0 Page 4 ESSENTIAL SAFETY NOTES There are a number of warning labels and symbols on your instrument. These are there to inform you

Version 1.0 Page 40 Calibration (Manual entry) Shows previously entered calibration values and allows values to be entered via the keypad. The

Version 1.0 Page 41 3: Bradford The procedure is as follows: Step 1Press 3 to select Bradford method. Step 2 Wa

Version 1.0 Page 42 Calibration Screen (replicates off) This shows the calibration values and allows standards to be measured. Step 11 In

Version 1.0 Page 43 Calibration (Manual entry) Shows previously entered calibration values and allows values to be entered via the ke

Version 1.0 Page 44 4: Lowry The procedure is as follows: Step 1Press 4 to select Lowry method. Step 2 Wavele

Version 1.0 Page 45 Calibration Screen (replicates off) This shows the calibration values and allows standards to be measured. Step 11 Inse

Version 1.0 Page 46 Calibration (Manual entry) Shows previously entered calibration values and allows values to be entered via the keypa

Version 1.0 Page 47 5: Biuret The procedure is as follows: Step 1Press 5 to select Biuret meth

Version 1.0 Page 48 Calibration Screen (replicates off) This shows the calibration values and allows standards to be measured. Step 11 Insert

Version 1.0 Page 49 Calibration (Manual entry) Shows previously entered calibration values and allows values to be entered via the keypad. The

Version 1.0 Page 5 INTRODUCTION Your spectrophotometer Your spectrophotometer is a simple-to-use UV/Visible instrument with a CCD array detec

Version 1.0 Page 50 Bacterial Cell Culture Measurement (OD600) • Bacterial cell cultures are routinely grown until the absorbance at 600 nm

Version 1.0 Page 51 Results ScreenStep 8 Insert the reference and press the 0A/100%T key. This will be used for all subsequent samples un

Version 1.0 Page 52 FAVORITES AND METHODS FOLDERS These folders are the storage locations for any user modified Applications (Methods) that ar

Version 1.0 Page 53 UTILITIES FOLDER 1 Set correct time and date 2 Select preferred language and number format 3 Printer/output o

Version 1.0 Page 54 Utilities 1: Date and Time The procedure is as follows: 2: Regional Sets Language and Number Format The procedure is as

Version 1.0 Page 55 4: Preferences Sets user preferences The procedure is as follows: 5: Contrast Ambient temperature can affect the displa

Version 1.0 Page 56 7: About 8: Games 1: Spectroblocks Classic block dropping game. Follow the instructions! Press Cancel to retur

Version 1.0 Page 57 2: Sudoku Can be set up as Computer mode (50 preset games) or User mode (enter your own pattern) Use the cursors to sele

Version 1.0 Page 58 ACCESSORIES INSTALLATION Printer installation 1. REMOVE THE POWER CABLE FROM THE INSTRUMENT. Turn the instrument over

Version 1.0 Page 59 Switch the instrument on and go to utilities/instrument/preferences and select the Built-in printer.

Version 1.0 Page 6 Keypad and display The back-lit liquid crystal display is very easy to navigate around using the alphanumeric entry and nav

Version 1.0 Page 60 Loading / changing the printer paper 1. Lift off the paper cover. Lock the platen and turn the knob to fe

Version 1.0 Page 61 Bluetooth accessory installation 1. REMOVE THE POWER CABLE FROM THE INSTRUMENT. Turn the instrument over and remove th

Version 1.0 Page 62 A B 5. Note the slots in the accessory cover, designed to engage with the Bluetooth accesso

Version 1.0 Page 63 9. Switch the instrument on and go to the preferences page under utilities/instrument, and select the Bluetooth option.

Version 1.0 Page 64 PRINT VIA COMPUTER • PVC (Print Via Computer) is a small application running under Windows 2000™ or Windows XP™ to enable

Version 1.0 Page 65 ACCESSORIES USB cable(spare) source locally Built-in printer accessory 80-3003-84 Bluetooth accessory 80-3003-96 SD Car

Version 1.0 Page 66 SPECIFICATION AND WARRANTY Wavelength range 190 - 1100 nm Monochromator Flat grating Wavelength calibration Automatic upon

Version 1.0 Page 7 Options (select using key pad numbers) 1. View parameters for the experiments. 2. Print the results. 3,4,5,6 Described i

Version 1.0 Page 8 Software style The user interface is built around having folders of files which are displayed on the home page when the ins

Version 1.0 Page 9 THE APPLICATIONS FOLDER 1 Absorbance or %T (Transmission) at a single user defined wavelength. 2 Concent