Hoefer SE215 Manuel d'utilisateur

user manualum    SE215-IM/Rev.L0/08-12Hoefer Multiple Gel CastersSE215, SE235, and SE275

Stacking gel preparationAdd a stacking gel to as many gels as required either individually to each gel after it is trans-ferred to the electrophoresis

Linear gradient gelsGradient gels are prepared by first pumping the “light” (lower % acrylamide) solutions and then gradually adding a “heavy” (higher

 gel caster  required volume   gradient maker    range (ml)   size* SE215 55 –100 SG100 SE235 32–56 SG50 or SG100 SE275 22– 40 SG30 or SG50*Actu

6Mix the gradient and pump the solution into the caster. While the solution is stirring, begin pumping from the mixing chamber and open the stopcock t

Note:  Do not store gels in the caster because they will be difficult to remove.After Gel Polymerization1To remove combs (if necessary): cover each san

4. Care and maintenanceClean the unit with soap and water after each use and rinse thoroughly with distilled water. Never use abrasive cleansers. Do n

• p145. Troubleshooting problem  solutionPlates broken Stack may be too tall. Before clamping, check that the stack is about 1 mm higher than the to

• p15problem  solutionSample wells damaged or leak Remove air pockets before inserting combs. Slide comb into solution at an angle. If comb must be

• p166. Ordering information product  quantity  code numberGel CastersFor 10 × 8 cm gelsSE275 4-Gel Caster, complete (10 × 8 cm), 2–4 gels. SE275

• p17product  quantity  code numberClampsRed clamps with springs, 4/pk SE252 for use on SE250, SE260 and on gel casters. Spacers  thickness  wid

• piContents 1. Multiple Gel Caster function ...12. Unpacking ...23.

• p18Combs         no. of wells  thickness (mm)  width (mm)   quantity  code number 5 0.75 13.0 1 SE211A-5-.75 5 1.00 13.0 1 SE211A-5-1.0 5

Hoefer, Inc. 84 October Hill Road Holliston, MA 01746Toll Free: 1-800-227-4750 Phone: 1-508-893-8999 Fax: 1-508-893-0176 E-mail: [email protected]

1. Multiple Gel Caster functionMultiple gel casters prepare gels that are for use in a Hoefer® miniVE, SE250 or SE260 gel electrophoresis unit. The ca

Note:  Wear gloves to keep the caster and plates free of finger marks. 2. UnpackingUnwrap all packages carefully and compare contents with the packing

• p33Single-concentration gels — Plug the red inlet port near the bottom of the faceplate with the black vinyl cap included with the unit. Check that

Fig 4. Stack components(Load from bottom up; Fillers are added after sandwiches are in place to adjust stack height)• Polycarbonate sheet. (Acts as l

4If pouring fewer gels than the capacity of the caster, lift up the polycarbonate sheet at the bottom of the stack and slide in fillers such as the acr

Prepare the monomer solution1Prepare enough monomer solution to fill all sand-wiches to the level of the notched plate (or lower if a stacking gel or fi

2Deaerate the monomer solution and add the initiator and catalyst just prior to pouring the gel. Fill the solu-tion into only one sandwich, allowing t